A phosphoprotein phosphatase from ox brain

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A phosphoprotein phosphatase from ox brain.

1. The pyrophosphate-exchange reactions which are catalysed by rat-liver preparations and depend upon leucine or isoleucine are profoundly modified by 'soluble' ribonucleic acid and by changes in magnesium concentration. The preponderant influence is exerted by the terminal nucleotide sequence of the 'soluble' ribonucleic acid. Lysinedependent pyrophosphate exchange occurs only at relatively hi...

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Purification and properties of phosphoprotein phosphatase from ox spleen.

Mangold, E. & Schmitt-Krahmer, C. (1927). Biochem. Z. 191, 411. McDonald, I. W. (1948a). Ph.D. Thesis, University of Cambridge. McDonald, I. W. (1948 b). J. Physiol. 107, 21P. McDonald, I. W. (1952). Biochem. J. 51, 86. McNaught, M. L. & Smith, J. A. B. (1947). Nutr. Abetr. Rev. 17, 18. Miller, J. I. & Morrison, F. B. (1942). J. Anim. Sci. 1, 353. Mitchell, H. H. & Hamilton, T. S. (1929). The B...

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Phosphoprotein phosphatase, a new enzyme from the frog egg.

In the nutrition of the young, the r&e of phosphoproteins is, without doubt, an important one. As casein in milk, or as the yellow yolk of many vertebrate and invertebrate eggs, phosphoprotein occurs in substantial quantities. In the frog egg, for example, the yolk platelets occupy almost half the volume of the cell. According to McClendon (5), the yolk of the frog egg consists of phosphoprotei...

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Purification of soluble arylsulphatases from ox brain.

1. Two soluble arylsulphatases (A and B) have been extracted from ox brain by a modified Albers autolysis method and purified by acetone and ammonium sulphate precipitation and dialysis. 2. A 1600-fold purification was achieved with arylsulphatase A and 320-fold purification with arylsulphatase B. 3. The specific activity of arylsulphatase A was 266000 4-nitrocatechol units/mg. of protein N, an...

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A specific protein carboxyl methylesterase that demethylates phosphoprotein phosphatase 2A in bovine brain.

Phosphoprotein phosphatase 2A (PP2A) is one of the four major protein serine/threonine phosphatases found in all eukaryotic cells. We have shown that the 36-kDa catalytic subunit of PP2A is carboxyl methylated in eukaryotic cells, and we have previously identified and purified a novel methyltransferase (MTase) that is responsible for this modification. Here, we describe a novel protein carboxyl...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1961

ISSN: 0006-2936

DOI: 10.1042/bj0810339